The Supercritical Co2 Advantage

What technology do we use?

Tispa Medical’s proprietary histopathology process replaces toxic solvents like xylene with CO2. This is a reclaimed gas with unique features when pressure and temperature are adjusted to induce a supercritical phase.

A process time of only 4 hours for gross cut fatty tissues, like mammary and colon is achieved using a powerful mixture of supercritical CO2 and ethanol. No pre- or post-treatment of  tissues is required, the processed tissues maintain cell morphology at new levels.

The timed release of ethanol into a retort, pressurized with CO2 enables the two to combine and dissolve into each other. Together they operate as a powerful solvent with low viscosity enabling a high diffusion rate and low surface tension. Once the ethanol and water are flushed out, paraffin is released in the retort to complete the process in minimal time.

co2 phase diagram
Supercritical CO2 has:
  • High density and high solvent power.
  • Low viscosity enabling high mass transfer rates.
  • No surface tension, which leads to high pore penetration rates

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